Varicella Zoster Virus (VZV) PCR, Ocular Fluids
| Label Mnemonic: | VZVQPCR |
| Epic code: | LAB8795 |
| Downtime form: | Doctor/Provider Orders - Pathology Core and Specialty Care Nursery |
Commercial Mailout Laboratory
6240-8 RCP
356-8593
6240-8 RCP
356-8593
Specimen(s):
Aqueous Fluid or Vitreous Fluid
Collection Medium:
| Sterile container |
Minimum:
Preferred Minimum: 0.1 mL Aqueous Fluid or Vitreous Fluid
Absolute Minimum: 0.05 mL Aqueous Fluid or Vitreous Fluid
Absolute Minimum: 0.05 mL Aqueous Fluid or Vitreous Fluid
Rejection Criteria:
Do not add water, saline or other fluid media to container. Do not
ship specimen in syringe.
Turn Around
Time:
Within 8 - 12 hours upon receipt at reference laboratory
Comments:
VZV reactivation is commonly seen in immunocompromised individuals.
These patients are more likely to have disseminated disease with
extensive skin lesions, pneumonia, hepatitis, or encephalitis. Proper
management is dependent upon early diagnosis; quantitative DNA PCR is
a rapid and sensitive tool useful for detecting the virus, tracking
the course of the infection, and monitoring response to
treatment.
The primers and probes used in this assay are specific for VZV based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against adenoviruses, BKV, CMV, EBV, HSV-1, HSV-2, HHV-6 variant A, HHV-6 variant B, HHV-7, HHV-8, JCV, parvovirus B19, and SV-40.
The primers and probes used in this assay are specific for VZV based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against adenoviruses, BKV, CMV, EBV, HSV-1, HSV-2, HHV-6 variant A, HHV-6 variant B, HHV-7, HHV-8, JCV, parvovirus B19, and SV-40.
Methodology:
Extraction of varicella-zoster viral DNA from specimen followed by
amplification and detection using real-time, quantitative PCR.
CPT Code:
87799