F-Actin (Smooth Muscle) Antibody, IgG with Reflex to IgG Titer

Label Mnemonic:	ACTIN
Epic code:	LAB8686
Order form:	Epic Req
Downtime form:	A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery

Commercial Mailout Laboratory
6240-8 RCP
356-8593

Specimen(s):

Serum

Collection Medium:

Red top tube 5 mL (Clot Activator)

Minimum:

Preferred Minimum: 1 mL serum
Absolute Minimum: 0.25 mL serum

Rejection Criteria:

Plasma. Contaminated, heat-inactivated, hemolyzed, or lipemic specimens.

Turn Around Time:

1-5 days upon receipt at reference laboratory

Reference Range:

F-Actin (Smooth Muscle) Ab, IgG by ELISA
19 Units or less
  Negative

20-30 Units
  Weak Positive - Suggest repeat testing in two to three weeks with
  fresh specimen.

31 Units or greater
  Positive - Suggestive of autoimmune hepatitis or chronic active
  hepatitis.

Smooth Muscle Antibody, IgG Titer
Less than 1:20
  Negative - No Antibody detected.

1:20-1:80
  Weak Positive - Suggest repeat testing in two to three weeks with
  fresh specimen.

1:160 or greater
  Positive - Suggestive of autoimmune hepatitis or chronic active
  hepatitis.

Interpretive Data:

Refer to report.

Comments:

In viral hepatitis the titers are generally less than 1:80 and are transient. The titers in primary biliary cirrhosis are also low, ranging from 1:20 to 1:40.

If F-Actin is 20 Units or greater, then Smooth Muscle Ab, IgG IFA titer will be added. Additional charges apply.

Methodology:

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody

CPT Code:

83516; if titer performed, 86256