Thermal Amplitude
| Label Mnemonic: | THERMAL AMPL |
| Epic code: | LAB7416 |
| Downtime form: | A-1a Doctor-Provider Orders - Pathology Blood Center |
DeGowin Blood Center - Blood Bank
C271 GH
356-2561
C271 GH
356-2561
Specimen(s):
Plasma
Collection Medium:
 | and | |
| Pink top tube 6 mL (K2-EDTA) | Pink top tube 6 mL (K2-EDTA) |
Minimum:
Contact Blood Bank prior to sample collection to obtain an insulated sample transport container.
Draw 2 full 6 mL pink stopper (EDTA) tubes. Place the samples in the insulated transport container and deliver to Blood Bank.
Draw 2 full 6 mL pink stopper (EDTA) tubes. Place the samples in the insulated transport container and deliver to Blood Bank.
Rejection Criteria:
Specimens that have not been scanned in Epic will be rejected.
Specimens not received in an insulated transport container obtained
from Blood Bank will be rejected. Samples collected with a neutral gel
separator will be rejected.
Delivery Instructions:
Keep specimen warm; deliver to laboratory immediately.
Refer to "Comments" for additional information.Testing Schedule:
0700-1400 Monday through Friday. For additional services, contact
Clinical Pathology Resident on-call at pager #3404.
Turn Around
Time:
24 hours (upon receipt in laboratory)
Reference Range:
Not applicable
Interpretive Data:
IgM cold-reactive autoagglutinins associated with immune hemolysis
usually react at temperatures greater than or equal to 30°C
and may have a titer of greater than or equal to 1000 when tested at
4°C. In-vitro thermal amplitude studies can be performed to
predict the likelihood of in-vivo hemolysis. The Thermal Amplitude
Study is used to determine the highest temperature that the
autoagglutinins react (specimen is tested at 37°C,
30°C, 20-24°C, and 1-6°C), the titer of the antibody at each temperature and the identity of the antibody.
30°C, 20-24°C, and 1-6°C), the titer of the antibody at each temperature and the identity of the antibody.
Comments:
Contact Blood Bank prior to sample collection to obtain an insulated
sample transport container. Collect in TWO properly labeled 6 mL pink
stopper EDTA tubes. Place the samples in the insulated transport
container (37°C) and deliver to Blood Bank ASAP. All Blood Bank
samples must be scanned in Epic.
If the only concern is for antibodies that react at 4°C, a Cold Agglutinin Titer (LAB4344) should be ordered.
If the only concern is for antibodies that react at 4°C, a Cold Agglutinin Titer (LAB4344) should be ordered.
Methodology:
Serial dilution tube test
CPT Code:
86886
See Additional Information:
Blood Center Specimen Collection
Blood Center Specimen Collection