Thermal Amplitude
Label Mnemonic: THERMAL AMPL
Epic Lab Code: LAB7416
Downtime Form: A-1a Blood Center Request
DeGowin Blood Center - Blood Bank
C271 GH
356-2561
Specimen(s):
Plasma
Collection Medium:
and
Pink top tube 6 mL (K2-EDTA) Pink top tube 6 mL (K2-EDTA)
Minimum:
Collect plasma in TWO 6 mL pink top tubes
Rejection Criteria:
Specimens that have not been scanned in Epic will be rejected. Specimens not received in an insulated transport container obtained from Blood Bank will be rejected. Samples collected with a neutral gel separator will be rejected.
Delivery Instructions:
Keep specimen warm; deliver to laboratory immediately. Refer to "Comments" for additional information.
Testing Schedule:
0700-1400 Monday through Friday. For additional services, contact Clinical Pathology Resident on-call at pager #3404.
Turn Around Time:
24 hours (upon receipt in laboratory)
Reference Range:
Not applicable
Interpretive Data:
IgM cold-reactive autoagglutinins associated with immune hemolysis usually react at temperatures greater than or equal to 30°C and may have a titer of greater than or equal to 1000 when tested at 4°C. In-vitro thermal amplitude studies can be performed to predict the likelihood of in-vivo hemolysis. The Thermal Amplitude Study is used to determine the highest temperature that the autoagglutinins react (specimen is tested at 37°C,
30°C, 20-24°C, and 1-6°C), the titer of the antibody at each temperature and the identity of the antibody.
Comments:
Contact Blood Bank prior to sample collection to obtain an insulated sample transport container. Collect in TWO properly labeled 6 mL pink stopper EDTA tubes. Place the samples in the insulated transport container (37°C) and deliver to Blood Bank ASAP. All Blood Bank samples must be scanned in Epic.

If the only concern is for antibodies that react at 4°C, a Cold Agglutinin Titer (LAB4344) should be ordered.
Methodology:
Serial dilution tube test
CPT Code:
86886
 
See Additional Information:
Blood Center Specimen Collection