Bone Marrow Aspirate

Adults: 3 mL Bone Marrow Aspirate. Testing requires a dedicated collection tube.

Room temperature for up to 24 hours, then refrigerate the whole blood if it is necessary to be held overnight, weekends or holidays.

Weekly

7-10 working days

Mutation not detected

By ordering this test the physician directs the molecular pathology laboratory to perform testing reflexively for the mutations commonly associated with polycythemia vera. JAK2 exons 12-14 and MPL exon 10 coding sequences are amplified by polymerase chain reaction (PCR) and sequenced in both directions using next generation sequencing. The JAK2 V617F mutation, or mutations in JAK2 Exons 12-14, and MPL exon 10 are reported in that order; once a mutation is identified no further analysis is performed (See the Polycythemia vera Algorithm). The reference sequences with which this patient's DNA sequence are compared are NM_004972, and NM_005373.1.

Allele specific real-time Polymerase Chain Reaction

The appropriate CPT code(s) will be billed depending on the patient's results including; 81270, and 81403 times 1 or times 2.