JAK2 Reflex for Polycythemia with Interpretation
Label Mnemonic: RFLX JAK2 PV
Epic Lab Code: LAB8540
Downtime Form: A-1a Molecular Pathology/Diagnostics Laboratory Requisition
Molecular Pathology
6004 BT GH
384-9568
Specimen(s):
Whole Blood
Collection Medium:
Pink top tube 6 mL (K2-EDTA)
Minimum:
Adults: 3 mL whole blood. Testing requires a dedicated collection tube.
Delivery Instructions:
Room temperature for up to 24 hours, then refrigerate the whole blood if it is necessary to be held overnight, or weekends, or holidays.
Testing Schedule:
Weekly
Turn Around Time:
7-10 working days
Reference Range:
Mutation not detected
Comments:
By ordering this test the physician directs the molecular pathology laboratory to perform testing reflexively for the mutations commonly associated with polycythemia vera. JAK2 exons 12-14 and MPL exon 10 coding sequences are amplified by polymerase chain reaction (PCR) and sequenced in both directions using next generation sequencing. The JAK2 V617F mutation, or mutations in JAK2 Exons 12-14, and MPL exon 10 are reported in that order; once a mutation is identified no further analysis is performed (See the Polycythemia vera Algorithm). The reference sequences with which this patient's DNA sequence are compared are NM_004972, and NM_005373.1.
Methodology:
Massively parallel next generation sequencing on DNA
CPT Code:
The appropriate CPT code(s) will be billed depending on the patient's results including; 81270, and 81403 times 1 or times 2.