ALK Fusion with Interpretation
Label Mnemonic: ALK FUSION
Epic code: LAB8566
Downtime form: A-1a Doctor/Provider Orders - Pathology Molecular
Molecular Pathology
6004 BT GH
Formalin-fixed, paraffin embedded tissue block or ten unstained 4 micron-thick sections on non-plus slides and one H&E stained section
Rejection Criteria:
Specimens that have been decalcified will not be accepted.
Turn Around Time:
10-14 days
Reference Range:
ALK Fusion not detected
Tissue sections are reviewed by a pathologist. RNA is isolated from the selected area of the sample. Anchored multiplex PCR for targeted next-generation sequencing is performed. The sequenced sample is a reverse transcription PCR-amplified fragment library in which each sample is uniquely identified by ligation of a short oligonucleotide barcode. The panel targets gene rearrangements of ALK1 and the resultant sequence identifies the exons of the fusion transcript arising from it and the partner gene. Fusion variants are detected from targeted ALK exons 19, 20, 21, 22, and 23 (reference sequences: NM_004304).
Massively parallel next generation sequencing on RNA
CPT Code: