EWSR1 Fusion
Label Mnemonic: EWSR1 FUSION
Epic code: LAB8309
Downtime form: A-1a Doctor/Provider Orders - Pathology Molecular
Molecular Pathology
6004 BT GH
Formalin Fixed, Paraffin Embedded Tissue Block
10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue
Tumor cells more than 50% of the total tissue and greater than 10mm2 in surface area on the block.
Rejection Criteria:
Specimens fixed in B5 fixative or that have been decalcified will not be accepted. Tumor specimens containing less than 50% tumor cells or are less than 10mm2 in area may be unacceptable.
Delivery Instructions:
Testing is generally not available on a STAT basis. However, expedited testing can be arranged by contacting the Molecular Pathology Laboratory at 384-9568.
Testing Schedule:
Turn Around Time:
21 days
RNA from FFPE tissue is used for amplification and massively parallel next generation sequencing to detect and identify EWSR1 fusion transcripts derived from gene arrangements that are associated with Ewing sarcoma, peripheral neuroectodermal tumor, clear cell sarcoma, desmoplastic round cell tumor (DRCT) and extraskeletal myxoid chondrosarcoma.
Massively parallel Next Generation RNA sequencing by synthesis, semiconductor technology, Ion Torrent, S5XL.
CPT Code: