Sarcoma Fusion Panel with Interpretation
Epic code: | LAB8427 |
Downtime form: | A-1a Doctor/Provider Orders - Pathology Molecular |
Molecular Pathology
6240 RCP
384-9568
6240 RCP
384-9568
Specimen(s):
Formalin Fixed, Paraffin Embedded Tissue Block
10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue
10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue
Minimum:
Tumor cells more than 50% of the total tissue and greater than 10mm2
in surface area on the block.
Rejection Criteria:
Specimens fixed in B5 fixative or that have been decalcified will not
be accepted. Tumor specimens containing less than 50% tumor cells or
are less than 10mm2 in area may be unacceptable.
Delivery Instructions:
Testing is generally not available on a STAT basis. However, expedited
testing can be arranged by contacting the Molecular Pathology
Laboratory at 384-9568.
Testing Schedule:
Weekly
Turn Around
Time:
21 days
Comments:
The Sarcoma Fusion Panel uses massively parallel next generation RNA
sequencing that can detect and identify fusions of 24 genes associated
with soft tissue cancers.
Gene Fusions Assessed (Those in bold may be ordered individually):
Gene Fusions Assessed (Those in bold may be ordered individually):
Disease Gene Aneurysmal bone cyst USP6 Adamantinoma NCOA2 Alveolar rhabdomyosarcoma (FKHR) FOXO1 Alveolar soft part, RCC TFE3 Chondroid lipoma MKL2 Chordoma HMGA2 Congenital fibrosarcoma NTRK3 Dermatofibrosarcoma protuberans PDGFB Endometrial stroma tumor JAZF1 Endothelial stromal sarcoma (PHF1) EPC1 Epithelioid hemangioendothelioma CAMTA1 Endometrial Stromal Sarcoma MEAF6 ESS, clear cell sarcoma of the kidney (FAM22) YWHAE Ewing/PNET, clear cell, DRCT, EWSR1 extraskeletal myxoid chondrosarcoma Ewing-like sarcoma (BCOR-CCNB3) CCNB3 Extrapleural solitary fibrous tumor STAT6 Extraskeletal Myxoid Chondrosarcoma TCF12 Extraskeletal myxoid chondrosarcoma TFG Extraskeletal Myxoid Chondrosarcoma, leukemia TAF15 Inflammatory myofibroblastic tumor ALK LG fibromyxoid, myxoid liposarcoma FUS Lipoblastoma PLAG1 Pericytoma, LG (ACTB) GLI1 Pigmented villonodular synovitis (COL6A3) CSF1 Synovial Sarcoma SS18 Undifferentiated small cell sarcoma (DUX4) CIC
Methodology:
Massively parallel Next Generation RNA sequencing by synthesis,
semiconductor technology, Ion Torrent, Personal Genomics Machine.
CPT Code:
81445