HLA-A,B Low Resolution - Platelet Refactory
Label Mnemonic: HLALRESAB
Epic Lab Code: LAB8515
Downtime Form: A-1a Miscellaneous Request
Commercial Mail-out Laboratory
5231 RCP
356-8593
Specimen(s):
Whole Blood or Buccal Swab
Collection Medium:
and or
Pink top tube 6 mL (K2-EDTA) Pink top tube 6 mL (K2-EDTA) Buccal Swab
Minimum:
Collect TWO full Pink top tubes (EDTA). This test needs its own dedicated tubes and cannot be shared with any other test.

Pediatrics Minimum: 4 mL
Adult Minimum: 5 mL

Buccal swab collection recommended if WBC <2.0 k/mmm3.
Collect: 4 Buccal swab samples. Swab collection kit available in Specimen Control (356-3527) or Mailouts (356-8593, M-F, 8-5).

Pediatrics Minimum: 3-4 swabs

Buccal Swab Collection Instructions:
1.  Collection kit contains 2 sterile cotton tipped applicator
    packages.  Each package contains 2 sterile cotton tipped swabs
   (applicators) for a collection kit total of 4 swabs.
2.  Four areas of the inner checks are swabbed:  upper left, lower
    left, upper right and lower right.  Use 1 cotton tipped swab for
    each area.
3.  Peel package open and grasp the shaft of the swab.  The sterile
    cotton swab tip should not touch anything except the inner check.
    Remove 1 swab at a time from the package.
4.  Swallow once.
5.  Open mouth, insert swab, and rub cotton tip vigorously against the
    inner check for 15 seconds.  
6.  Insert the swab as shown in the brochure into the foam holder.
    The swab can now touch the brochure paper.
7.  Repeat steps 3 - 6 for the three remaining swabs.
8.  After inserting all 4 swabs into the foam holder, peel off the
    adhesive cover to the left and fold this covering flap up over the
    swabs.
9.  Label brochure with patient identification/specimen collection
    label.  
10. Place brochure in envelope and send to Specimen Control.
Delivery Instructions:
Keep at room temperature. Do not refrigerate. Deliver to laboratory immediately after collection.
Turn Around Time:
5-7 days upon receipt at reference laboratory
Comments:
This test is used for patients with evidence of alloimmune platelet transfusion refractoriness and should NOT be used to determine HLA type of a patient who is being evaluated for hematopoietic stem cell transplant. If the patient has been transfused, ideally this test should not be drawn until 48 hours post transfusion. Please print, complete and submit the Histocompatibility Non- Transplant Test Reqisition Form from the Blood Center of Wisconsin with the specimen and the A-1a Miscellaneous Request.
Methodology:
PCR-rSSO
CPT Code:
81373 x2