AFB Blood Culture
Label Mnemonic: AFBBL
Epic Lab Code: LAB246
Downtime Form: A-1a Clinical Microbiology Laboratory Requisition
BT 6004
Bacteriology/Virology Section
Specimen Instructions:
5 mL (maximum) per bottle
Collection Medium:
Myco/F Lytic Bottle - available from the Microbiology Laboratory
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
6 weeks
Myco/F Lytic vials are not routinely used for blood culture as routine blood cultures are effective for most pathogens. Yield of culture is critically affected by the volume of blood drawn: at minimum, THREE bottles from 2 venipunctures and ideally SIX bottles from 3 venipunctures should be obtained. Each bottle should be filled with 5 mL of blood. Bottles are available from the Microbiology laboratory (356-2591). The following guidelines were developed specifically for the diagnosis of M. chimaera endovascular infection, where bacterial loads are likely high. 1. Order one "AFB Blood Culture R/O Mycobacterium chimaera" in EPIC for each venipuncture (LAB8454). Obtain Isolator tubes sized for pediatric patients (1.5 mL) or adults (10 mL) from Hospital Stores or nearby clinic area if in stock. •1.5 mL; pediatric Isolator blood culture tube (Hospital Stores 923003) •10 mL; adult Isolator blood culture tube (Hospital Stores 922848) 2. Follow instructions in Nursing policy N-03.020 Blood Culture Collection, Adult or PHL-06 Phlebotomy protocol for antisepsis and drawing blood. 3. ADULTS with suspected mycobacterial endocarditis: collect 2 venipunctures (2 orders total) with 1 Isolator tube per venipuncture site. An additional Isolator tube may be drawn to maximize diagnostic yield (3 orders total). PEDIATRIC patients with suspected mycobacterial endocarditis: obtain 1 or 2 venipunctures and pediatric and/or adult- sized Isolator tubes as below: ----------------------------------------------------------- AGE (years) 10 mL Tubes to Fill 1.5 mL Tubes to Fill 1 None 1 (1 venipuncture) 2 None 1 (1 venipuncture) 3 None 2 (2 venipunctures) 4 1 (1 venipuncture) None 5 1 (1 venipuncture) None 6 and above 2 (2 venipunctures) None ----------------------------------------------------------- 4. Inoculate either 1.5 mL or 10 mL into each Isolator tube: tubes MUST be full as over-concentrated substances within the tubes are toxic to bacteria. 5. Send to Microbiology laboratory; call 356-2591 with questions.
CPT Code:
See Additional Information:
Phlebotomy Team Policies