Blood Culture - Adult, Multiple Sets
Epic Lab Code: O120710 and O123906 (after visit)
Downtime Form: A-1a Doctor-Provider Orders - Pathology Microbiology
BT 6004
Bacteriology/Virology Section
Specimen Instructions:
Most blood cultures should be ordered as 3 sets (a set consists of a pair of aerobic and anaerobic bottles) of bottles containing 10 mL of blood each (60 mL total). To diagnose catheter-related bloodstream infection (CRBSI) please use the CRBSI order panel in Epic [O116026] instead of this order. Key principles for the success of blood culture are as follows: 1) Sensitivity is determined by the total blood volume collected, before antibiotics. a. 6 full (10 mL each) bottles will miss 2% of bloodstream infections. b. 4 full (10 mL) bottles will miss 13% of bloodstream infections. c. 2 full or 4 half-full bottles will miss 28% of bloodstream infections. d. Ideally, three venipunctures should be performed immediately but a third set of bottles can be drawn at a 4-6 hour delay without significant loss of yield. 2) False positive cultures are avoided by proper antisepsis and never drawing from indwelling catheters when a peripheral stick can be obtained. Multiple venipunctures assist with interpretation of culture contaminants.
Collection Medium:
Aerobic Blood Culture Bottle Anaerobic Blood Culture Bottle
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
Cultures are held for 5 days
[1] See Nursing Policy and Procedure (N-03.020).
[2] To maximize volume, refer to phlebotomy protocols PHL-6 and PHL-12.
Automated, continuous monitoring.
CPT Code:
See Additional Information:
Pediatric Maximum Blood Volume Guidelines