IDH1 & IDH2 Mutation Analysis
Label Mnemonic: IDH12
Epic Lab Code: LAB8289
Downtime Form: A-1a Molecular Pathology/Diagnostics Laboratory Requisition
Molecular Pathology
6004 BT GH
384-9568
Specimen(s):
Peripheral Blood
Specimen Instructions:
Testing requires dedicated collection tube.
Collection Medium:
Pink top tube 6 mL (K2-EDTA)
Minimum:
Collect 3 mL blood in Pink top tube.
Testing Schedule:
Weekly
Turn Around Time:
21 days
Comments:
Sequence analysis is performed for exons 4 and 6 of IDH1 and exon4 of IDH2 and includes detection of the common variants; IDH1 p.R132C (394C>T), IDH2 p.R140Q (c.419G>A), and IDH2 p.R172K (c.515G>A). These variants may be detected in several different tumor types. However, peripheral blood and bone marrow testing is primarily indicated for patients with hematologic malignancy including acute myeloid leukemia.
Methodology:
DNA sequencing by synthesis, semiconductor technology, Ion Personal Genomics Machine
CPT Code:
81403 - IDH1, 81403 - IDH2