Nicotine Metabolite, Rapid Screen
Label Mnemonic: UCOT
Epic code: LAB8071
Downtime form: A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
6240 RCP
Collection Medium:
Clear top tube
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
6.0 mL random urine, no preservative in clear top tube
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
Test cut-off: 300 ng/mL cotinine (nicotine metabolite). Positive results for this assay indicate active tobacco use within 1 week of testing.
Interpretive Data:
Cotinine is one of the main metabolites of nicotine and is excreted in urine with tobacco or nicotine patch use. Cotinine has an elimination half-life of approximately 15 hours in adults. Urine cotinine concentrations exceeding 1,000 ng/mL indicate active use of tobacco or high-dose nicotine patch. Non-users of tobacco or those with only passive exposure to tobacco smoke have urine cotinine concentrations well below 300 ng/mL (typically less than 20 ng/mL).
Moyer TP, Charlson JR, Enger RJ, et al: Simultaneous analysis of nicotine, nicotine metabolites, and tobacco alkaloids in serum or urine by tandem mass spectrometry, with clinically relevant metabolic profiles. Clin Chem 2002;48:1460-1471.
Test Limitations:
1. A positive result from this assay indicates only the presence of cotinine and does not necessarily correlate with the extent of physiological and psychological effects.

2. The test is designed for use with human urine only.

3. It is possible that other substances and/or factors, e.g., technical, procedural issues or other cotinine-like compounds other than those investigated in the specificity study, may interfere with the test and cause false results.

4. For more detailed profiling of nicotine and tobacco product metabolites, analysis by mass spectrometry-based method is required.
Enzyme Immunoassay
CPT Code: