Nicotine Metabolite, Rapid Screen
|Clear top tube
Call laboratory for additional acceptable specimen collection containers.
6.0 mL random urine, no preservative in clear top tube
24 hrs/day, 7 days a week, including holidays.
1 hour (upon receipt in laboratory)
Test cut-off: 300 ng/mL cotinine (nicotine metabolite). Positive
results for this assay indicate active tobacco use within 1 week of
Cotinine is one of the main metabolites of nicotine and is excreted in
urine with tobacco or nicotine patch use. Cotinine has an elimination
half-life of approximately 15 hours in adults. Urine cotinine
concentrations exceeding 1,000 ng/mL indicate active use of tobacco or
high-dose nicotine patch. Non-users of tobacco or those with only
passive exposure to tobacco smoke have urine cotinine concentrations
well below 300 ng/mL (typically less than 20 ng/mL).
Moyer TP, Charlson JR, Enger RJ, et al: Simultaneous analysis of
nicotine, nicotine metabolites, and tobacco alkaloids in serum or urine
by tandem mass spectrometry, with clinically relevant metabolic
profiles. Clin Chem 2002;48:1460-1471.
1. A positive result from this assay indicates only the presence of
cotinine and does not necessarily correlate with the extent of
physiological and psychological effects.
2. The test is designed for use with human urine only.
3. It is possible that other substances and/or factors, e.g.,
technical, procedural issues or other cotinine-like compounds other
than those investigated in the specificity study, may interfere with
the test and cause false results.
4. For more detailed profiling of nicotine and tobacco product
metabolites, analysis by mass spectrometry-based method is