JAK2 Common Mutation V617F Analysis with Interpretation
Label Mnemonic: JAK2BM
Epic Lab Code: LAB8031
Downtime Form: A-1a Molecular Pathology/Diagnostics Laboratory Requisition
Molecular Pathology
6004 BT GH
384-9568
Specimen(s):
Bone Marrow Aspirate
Collection Medium:
Lavender top tube 3 mL (EDTA)
Minimum:
2 mL bone marrow aspirate
Delivery Instructions:
Room temperature for up to 24 hours, then refrigerate the whole blood if it is necessary to be held overnight, or weekends, or holidays.
Testing Schedule:
Weekly
Turn Around Time:
7 working days
Reference Range:
Direct detection of G-T transversion at position 1849 of the Janus kinase 2 locus, giving rise to a Valine-to-Phenylalanine substitution at position 617 (JAK2 V617F mutation).
Comments:
This test is useful for diagnosis of myeloproliferative disorders: Polycythemia Vera and Essential Thrombocythemia.
Methodology:
Allele specific real-time Polymerase Chain Reaction