MPL codon 515 Mutation
Label Mnemonic: MPL
Epic Lab Code: LAB8118
Downtime Form: A-1a Molecular Pathology/Diagnostics Laboratory Requisition
Molecular Pathology
6004 BT GH
384-9568
Specimen(s):
Blood
Collection Medium:
Pink top tube 6 mL (K2-EDTA)
Minimum:
Adults: 3 mL whole blood. Testing requires a dedicated collection tube.
Delivery Instructions:
Room temperature for up to 24 hours, then refrigerate the whole blood if it is necessary to be held overnight, or weekends, or holidays.
Testing Schedule:
Weekly
Turn Around Time:
7-10 working days
Interpretive Data:
Mutation not detected
Comments:
MPL exon 10 coding sequence is amplified by polymerase chain reaction (PCR) and sequenced in both directions using next generation sequencing. The reference sequence with which this patient's DNA sequence is compared is NM_005373.1. The test will detect codon 505 and 515 mutations.
Methodology:
Massively parallel next generation sequencing on DNA
CPT Code:
81403