Bronchial Lavage/Bronchial Wash: 2 mL, collected in sterile, 
screw-cap tube

CSF: 2 mL submitted in sterile, screw-cap tube

Tissue: Place a minimum of 5 mg fresh tissue (approximately 1/2 of a 
pencil eraser size) in a sterile, screw top container. Do not add 
water, saline or other fluid media to container. Formalin fixed, 
paraffin embedded tissue will not be accepted.

Tracheal Aspirate/Tracheal Wash: 2 mL collected in a sterile, screw top 
tube

HHV-7 is detectable in a variety of transplant settings, both HSCT 
and solid organ. Direct effects of HHV-7 include fever, rash, 
myelosuppression, encephalitis, and pneumonitis. Potentially more 
important are the indirect effects HHV-7 has on CMV disease, invasive 
fungal disease, and allograft dysfunction. Quantitative HHV-7 DNA PCR 
can be used to document the presence of the virus as well as track the 
course of infection.

The primers and probes used in this assay are specific for known 
strains of HHV-7 based on similarity search algorithms. Additionally, 
no cross reactivity was detected when tested against adenoviruses, BKV, 
CMV, EBV, HSV-1, HSV-2, HHV-6 variant A, HHV-6 variant B, HHV-8, JCV, 
parvovirus B19, SV-40, and VZV.