CEBPA Full Gene Sequence with Interpretation, Bone Marrow
Label Mnemonic: | CEBBM |
Epic code: | LAB7765 |
Downtime form: | Doctor/Provider Orders - Pathology Molecular |
Molecular Pathology
6240 RCP
384-9568
6240 RCP
384-9568
Specimen(s):
Bone Marrow Aspirate
Formalin fixed, paraffin-embedded tissue
Formalin fixed, paraffin-embedded tissue
Collection Medium:
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Pink top tube 6 mL (K2-EDTA) |
Minimum:
2 mL bone marrow aspirate. Specimens for which the AML blast count is
at least 20% will be tested. Testing on specimens with a lower blast
count may be attempted with approval of lab director. However, such
testing is not recommended due to the increased possibility of a false-
negative result.
Rejection Criteria:
Blast count less than 20%, hemolyzed, green top, decalcified bone
marrow.
Delivery Instructions:
Testing is generally not available on a STAT basis. However,
expedited testing can be arranged by contacting the Molecular
Pathology Laboratory at 384-9568 or the Molecular Genetic Pathology
Fellow (pager #8682).
Testing Schedule:
Weekly
Turn Around
Time:
7-10 working days
Reference Range:
Unaffected samples will lack disease-associated CEBPA
mutations, but may harbor previously identified single nucleotide
polymorphisms (SNPs) that are reported as incidental findings.
Test
Limitations:
20% mutant allele
Methodology:
PCR amplification of CEBPA-specific fragments followed by DNA
cycle sequencing (Sanger method).
CPT Code:
81218
References
1. Zhang P, et al. Immunity 2004;21(6):853-63.
2. Nerlov C. Nature Reviews Cancer 2004;4(5):394-400.
3. Dufour A, et al. J Clin Oncol 2010;28(4):570-7.
4. Wouters BJ, et al. Blood 2009;113(13):3088-91.
5. Taskesan E, et al. Blood 2011;117:2469-75.
6. Gombart AF, et al. Blood 2002;99(4):1332-1340.
7. Shih LY, et al. Clin Cancer Res 2005;11(5):1821-26.