KIT (GIST) Targeted Gene Analysis Exons 9, 11, 13, 17 with Interpretation
6004 BT GH
Formalin fixed, paraffin embedded tissue block or 1 H&E with 10
unstained slides 5-10 micron-thick on uncharged slides at 20-
Specimen requires 20% or greater tumor cell content. Submit 1 H&E
with 10 unstained 6 micron-thick sections on non-charged slides or
formalin-fixed, paraffin-embedded tissue block.
Specimens fixed in B5 fixative or that have been decalcified will not
be accepted. Tumor specimens containing less than 20% tumor cells or
are less than 10mm2 in area may be unacceptable.
Testing is generally not available on a STAT basis. However,
expedited testing can be arranged by contacting the Molecular
Pathology Laboratory at 384-9568 or the Molecular Genetic Pathology
Fellow (pager #8682).
0800-1700 Testing offered once per week
excluding weekends and University holidays.
7-10 working days
The principle use of this test is to detect mutations in patients with
gastrointestinal stromal tumor (GIST) and melanoma. For acute myeloid
leukemia (AML) testing, please order KITAML Mutation Analysis
(LAB7659). This test is not intended to detect the D816 mutation in
bone marrow for mast cell disease or to detect minimal residual
disease, see KITMAST Mast Cell Disease (LAB7567).
Massively parallel Next Generation DNA sequencing by synthesis,
semiconductor technology, Ion Personal Genomics Machine.