Neutrophil Oxidative Burst (DHR)
Epic Lab Code: SPL8
Downtime Form: A-1a Miscellaneous Request
VA Diagnostic Immunology Lab
Veterans Affairs Hospital
Rm. 223, Bldg. 41
319-338-0581, EXT 7550
dial 158 from UIHC
Specimen(s):
Whole Blood
Specimen Instructions:
Call VA Diagnostic Immunology Lab as soon as sample is drawn
(319-338-0581) ext. 7550.

Blood cannot be held longer than 8 hours before processing.

ATTENTION: For samples drawn in Medicine Specialty Clinics and Pediatric Specialty Clinics place in VA DIAG. IMM (Ballas) box.

For samples drawn on the floor please call the lab for pick up
(319-338-0581) ext. 7550.
Minimum:
10 mL; sodium heparin green top. Do not use a needle smaller than 21 gauge.
Delivery Instructions:
Keep at room temperature. Do not refrigerate. Do not centrifuge.
Testing Schedule:
This assay is run Monday through Friday 8 a.m. to noon.
Reference Range:
Internal control and normal range reported with each sample.
Comments:
This assay is run using viable lymphocytes.

This assay is used to determine whether the NADPH Oxidase of phagocytic cells in a certain individual is normal. This is done by flow cytometry using the probe dihydrorhodamine 123 (DHR). This assay replaces the Nitro Blue Tetrazolium test (NBT). This assay is the gold standard to rule out Chronic Granulomatous disease (CGD).