Hepatitis B Virus DNA, Ultra Sensitive Quantitative PCR
Label Mnemonic: HBVQUANT
Epic Lab Code: LAB3284
Downtime Form: A-1a Clinical Microbiology Laboratory Requisition
Microbiology/Molecular Infectious Disease
BT 6004
356-2591
Specimen(s):
Plasma
Specimen Instructions:
All collection tubes need to be processed within 24 hours of collection. If specimens cannot reach the Microbiology laboratory at University of Iowa Hospitals and Clinics within the 24 hours, process as follows:

Centrifuge for 20 minutes at 800-1600 x g. Aseptically enter the tube and aliquot into sterile tubes. Transport to the laboratory on ice within 24 hours.
Collection Medium:
Pink top tube 6 mL (K2-EDTA)
Minimum:
One full pink top tube EDTA anti-coagulated pink top tube. Sample tube must remain sterile. HBV testing cannot be added onto a previously opened Vacutainer® tube. Testing requires a dedicated collection tube. If also testing for HCV RNA or Genotype, collect a second Pink top tube (6 mL whole blood or 3 mL plasma).
Delivery Instructions:
Deliver to laboratory immediately after collection.
Testing Schedule:
Weekly
Reference Range:
Negative

Analytical range in log10 values:
  1.3 - 8.2 IU/mL (20-170,000,000 IU/mL non-log transformed values)

Positive results less than 20 IU/mL will be reported as "POS <1.3 LOG IU" and negative results will be reported as "Not detected".
Comments:
Testing only approved for viral load testing to monitor therapy. Not for diagnostic testing.
Methodology:
PCR amplification; Cobas HBV Test (Roche Diagnostics, Inc.)
CPT Code:
87517