Chlamydophila (Chlamydia) pneumoniae by PCR
Label Mnemonic: CPPCR
Epic Lab Code: LAB7517
Downtime Form: A-1a Miscellaneous Request
Commercial Mail-out Laboratory
5231 RCP
356-8593
Specimen(s):
Nasal Wash, Nasopharyngeal Swab, Bronchoalveolar Lavage (BAL), Pleural Fluid
Specimen Instructions:
Collect nasopharyngeal swab in viral transport medium (33595). Alternatively, collect tracheal aspirate, bronchoalveolar lavage, bronchial wash, or nasopharyngeal wash/aspirate in sterile, leak-proof container. Nasopharyngeal swab: Collect specimen using the flexible minitip flocked swab (Hospital Stores #33595). A. Measure the distance from the patient's nostril to the nasopharynx (half the distance from nostril to base of the ear) and hold the swab at that location. Do not advance the swab beyond that point. B. Gently insert the swab along the base of one nostril (straight back, not upwards) and continue along the floor of the nasal passage until reaching the nasopharynx. C. Rotate swab 2-3 times and hold in place for 5 seconds. Place swab in tube containing viral transport medium. Break off the excess length of swab at the score mark to permit capping of the tube. Nasopharyngeal wash/aspirate: A. Assemble equipment: Sterile specimen trap Personal Protective Equipment (gloves, surgical mask, eye protection) Appropriate size suction catheter (8 fr for infants/children, 10/12 fr for adults) Normal saline vial Wall suction Bag or cup of ice for specimen transport to laboratory B. Place patient with the head tilted slightly back. C. With sterile gloved hand, insert suction catheter into the patient's nose to the depth of the nasopharyngeal area (beyond the turbinates). Do not remove catheter until end of procedure. (see diagram) D. With the non-sterile gloved hand, instill approximately 1-2 mL normal saline outside the catheter. E. Apply suction to aspirate nasopharyngeal secretions. F. Above steps may need to be repeated to obtain 1 mL sample in specimen trap. G. Remove catheter from patient. With specimen trap still in-line, rinse catheter with remaining saline to clear secretions. H. Specimens transported by tube system must be transferred from trap to a leak-proof sterile container (be sure the lid is tightly secured).
Collection Medium:
Swab Kit Flexible Nasopharyngeal--Viral 3.0 mL
Minimum:
2 mL respiratory specimen in sterile container or swab. Samples submitted to Specimen Control will be transferred to UTM viral transport media.
Rejection Criteria:
Dry swabs and leaking or non-sterile containers. Respiratory aspirates in collection containers with tubing (sample tends to leak from these containers, compromising the specimen).
Turn Around Time:
5 days upon receipt at reference laboratory
Methodology:
Qualitative Polymerase Chain Reaction
CPT Code:
87486