Fluorescence In Situ Hybridization for Her-2/neu
Label Mnemonic: FCERB
Immunopathology
5238 RCP
356-2688
Specimen(s):
Formalin-fixed, Paraffin-embedded tissue
Specimen Instructions:
Tissue is to be fixed in 10% Neutral buffered formalin for at least 6 hours and no longer than 72 hours.
Minimum:
Only 10% formalin fixed paraffin blocks are accepted. Total time of tissue in formalin is required information and must be recorded on the requisition. If IHC Her2Neu results are available please send results, and corresponding slides if possible.
Turn Around Time:
Final results within 1 week
Reference Range:
The results are reported as a ratio of HER2 signals to chromosome 17 centromere signals.
1) A ratio >2.0 and/or HER2 copy number >6.0 is consistent with amplification of HER2.
2) A ratio of <2.0 and HER2 copy number >4.0 and <6.0 is a borderline finding and requires further testing.
3) A ratio of <2.0 and HER2 copy number <4.0 is considered not amplified. These results are reported per the 2013 ASCO/CAP guidelines.
Methodology:
Fluorescence In Situ Hybridization (FISH)
CPT Code:
88368