Label Mnemonic: AMPU
Epic code: LAB7381
Downtime form: A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
6240 RCP
Urine Screen
Collection Medium:
Clear top tube
Alternate Collection Media:
Urine (Random)-BD Vacutainer®, no additive yellow top
5 mL random urine
Rejection Criteria:
Test cannot be added to a urine sample previously utilized for urinalysis testing (LAB1160, LAB1164, LAB8379).
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
Interpretive Data:
Drug of abuse screening tests are to be used for medical purposes only and not for non-medical purposes (e.g., employee, competitive athlete, or forensic testing).
Screen includes amphetamines only.  For full drug of abuse-urine 
panel, see "Drug of Abuse Screen".

If confirmation is needed for amphetamines, call the Laboratory at 
319-356-3527.  Allow up to seven days for confirmatory results.  
Confirmation is at an additional charge.

Additional information on approximate cut-offs for individual drugs or 
drug metabolites in the specific assays

Drug                       Approximate cut-off for
                           amphetamines assay (ng/mL)
d-Amphetamine                        981
d-Methamphetamine                    998
MBDB                               1,175
MDA                                  771
MDEA                               1,553
MDMA ("Ecstasy")                     509
PMMA                                 690
PMA                                  908
Labetalol metabolite               5,116
Phendimetrazine*                 138,000
Phentermine*                     239,000
d-Pseudoephedrine*               261,000
Ephedrine*                       308,000

Abbreviations for the "designer" amphetamine and methamphetamine 
   MBDB - methylbenzodioxolylbutanamine ("Eden")
   MDA - 3,4-methylenedioxyamphetamine
   MDEA - 3,4-methylenedioxy-N-ethylamphetamine ("Eve")
   MDMA - 3,4-methylenedioxymethamphetamine ("Ecstasy")
   PMA - para-Methoxyamphetamine
   PMMA - para-Methoxymethamphetamine 

* The concentrations of these compounds needed to trigger a positive
  amphetamines screen are very high and likely only achievable in large

New amphetamines assay instituted 7/7/10.  
Unlike the assay used prior to 7/7/10, the new assay has very good 
cross-reactivity for MDMA (Ecstasy) and some designer amphetamines 
(MDA, MBDB, MDEA, PMA, PMMA).  The older assay did not cross-react 
well with amphetamines other than amphetamine and methamphetamine. The 
new assay has low cross-reactivity with non-amphetamine drugs 
(ephedrine, pseudoephedrine, phentermine, etc.).

Patients on labetalol can have a false positive amphetamines screen 
due to a metabolite of labetalol (APB, 1-Methyl-3-phenylpropylamine)
structurally resembling amphetamine. In these cases, confirmatory 
testing will be negative.


Belson MG, Simon HK, Sullivan K, Geller RJ.  The Utility of 
Toxicologic Analysis in Children with Suspected Ingestion.  Pediatr 
Emerg Care 1999;15:383-387.

Bast RP, Helmer SD, Henderson SR, Rogers MA, Shapiro WM, Smith RS.  
Limited Utility of Routine Drug Screening in Trauma Patients.  South 
Med J 2000;93:397-399.

Hammett-Stabler CA, Pesce AJ, Cannon DJ.  Urine Drug Screening in the 
Medical Setting.  Clinica Chimica Acta 2002;315:125-135.

Schiller MJ, Shumway M, Batki SL.  Utility of Routine Drug Screening 
in a Psychiatric Emergency Setting.  Psychiatric Services 2000;51:474-

Sugarman JM, Rodgers GC, Paul RI.  Utility of Toxicology Screening in 
a Pediatric Emergency Department.  Pediatric Emergency Care.  
Pediatric Emergency Care 1997;13(3):194-197.

Gilbert RB, Peng PI, Wong D.  A labetalol metabolite with analytical 
characteristics resembling amphetamines.  J Anal Toxicol 1995;19(2):84-
Test Limitations:
There is the possibility that other substances and/or factors may interfere with the test and cause erroneous results (e.g., technical or procedural errors). Call the lab at 319-356-3527 for additional information.
Assay is based on the kinetic interaction of microparticles in a solution (KIMS) as measured by changes in light transmission.
CPT Code:
See also:
Drugs of Abuse, Urine
Drugs of Abuse, Urine + Confirm