Staphylococcus aureus (MRSA/MSSA) by PCR
Label Mnemonic: SAPCR
Epic Lab Code: LAB5810
Downtime Form: A-1a Clinical Microbiology Laboratory Requisition
Microbiology/Molecular Infectious Disease
BT 6004
356-2591
Specimen(s):
Surveillance Swab Specimen collected from Nares
Specimen Instructions:
Copan Dual Swab (#26200) is available from Hospital Stores. With patient's head tilted back, insert both dry swabs (leave attached to red cap) approximately 1-2 cm into one nostril. Rotate the swabs against the inside of the nostril for 3 seconds. Apply slight pressure with a finger on the outside of the nose to ensure good contact between the swabs and inside of the nose. Using the same swabs, repeat for the other nostril. Place the swabs into the transport tube (swabs should stay attached to the red cap). Make sure the red cap is tightly on the transport tube. Transport: Room temperature up to 24 hours, otherwise refrigerate (2-8°C) up to 5 days.
Collection Medium:
Copan Dual Swab
Rejection Criteria:
Specimen collected using different swab (not Copan dual swab) or from site other than nares will be rejected.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
2 hours (upon receipt in laboratory)
Comments:
Used to detect colonization with SA and methicillin-resistant Staphylococcus aureus (MRSA). A positive result does not necessarily indicate viable organism.

The primers and probes in the Xpert SA Nasal Complete assay detects a proprietary sequence for the staphylococcal protein A (spa) gene, the gene for methicillin resistance (mecA), and the staphylococcal cassette chromosome mec (SCCmec) inserted into the SA chromosomal attB site. Inhibition of the SA Nasal Complete assay resulting in Invalid or false Negative test results has been observed in the presence of inhaled nasal steroids Flonase and Nasonex. Reliable results are dependent on proper specimen collection, handling, and storage.
Methodology:
PCR amplification; Xpert SA Test (Cepheid)
CPT Code:
87641