Staphylococcus aureus (MRSA/MSSA) by PCR
| Label Mnemonic: | SAPCR |
| Epic code: | LAB5810 |
| Downtime form: | Doctor/Provider Orders - Pathology Microbiology |
Microbiology/Molecular Infectious Disease
BT 6004
356-2591
BT 6004
356-2591
Specimen(s):
Surveillance Swab Specimen collected from Nares
Specimen
Instructions:
Copan Dual Swab (#26200) is available from Hospital Stores.
With patient's head tilted back, insert both dry swabs (leave attached
to red cap) approximately 1-2 cm into one nostril. Rotate the swabs
against the inside of the nostril for 3 seconds. Apply slight pressure
with a finger on the outside of the nose to ensure good contact
between
the swabs and inside of the nose. Using the same swabs, repeat for the
other nostril. Place the swabs into the transport tube (swabs should
stay attached to the red cap). Make sure the red cap is tightly on the
transport tube.
Transport: Room temperature up to 24 hours, otherwise refrigerate
(2-8°C) up to 5 days.Collection Medium:
) |
| Copan Dual Swab (Hospital Stores #26200) |
Rejection Criteria:
Specimen collected using different swab (not Copan dual swab) or from
site other than nares will be rejected.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
2 hours (upon receipt in laboratory)
Methodology:
PCR amplification; Xpert SA Test (Cepheid)
CPT Code:
87641
The primers and probes in the Xpert SA Nasal Complete assay detects a proprietary sequence for the staphylococcal protein A (spa) gene, the gene for methicillin resistance (mecA), and the staphylococcal cassette chromosome mec (SCCmec) inserted into the SA chromosomal attB site. Inhibition of the SA Nasal Complete assay resulting in Invalid or false Negative test results has been observed in the presence of inhaled nasal steroids Flonase and Nasonex. Reliable results are dependent on proper specimen collection, handling, and storage.