KRAS Gene Analysis Variants in codons 12 and 13 and Exon 3 with Interpretation
Label Mnemonic: KRAS
Epic Lab Code: LAB5763
Downtime Form: A-1a Miscellaneous Request
Molecular Pathology
6004 BT GH
384-9568
Specimen(s):
Formalin fixed, paraffin embedded tissue block and 10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue
Minimum:
Tumor cells more than 50% of the total tissue and greater than 10mm2 in surface area on the block.
Rejection Criteria:
Specimens fixed in B5 fixative or that have been decalcified will not be accepted. Tumor specimens containing less than 50% tumor cells or are less than 10mm2 in area may be unacceptable.
Testing Schedule:
Weekly
Turn Around Time:
7-10 working days
Reference Range:
Negative
Comments:
The tissue will be reviewed by a pathologist prior to testing to identify that it contains at least 50% tumor.

This assay detects mutations in codons 12, 13 and 61 (19 mutations in total).
Methodology:
Polymerase Chain Reaction and Single Nucleotide Primer Extension. Massively parallel Next Generation DNA sequencing by synthesis, semiconductor technology, Ion Personal Genomics Machine.
CPT Code:
81276