Fluorescence In-Situ Hybridization (FISH-Paraffin Embedded)

Epic code:	CYT58
Downtime form:	Cytogenetics Request

Shivanand R. Patil Cytogenetics & Molecular Laboratory
Department of Pediatrics
W-101 GH
356-3877 (Laboratory)

Specimen(s):

Paraffin Embedded Tumor Tissue

Minimum:

4 unstained slides on charged slide, 1 H&E slide with tumor marked. All slides from the same block cut sequentially.

Delivery Instructions:

Submit specimen to laboratory as soon as possible after collection. After hours specimens should be taken to Specimen Control. For questions after hours, call (319) 356-1616 and ask the operator to page the cytogenetics on-call staff at pager #5525.

Testing Schedule:

Specimens accepted in the lab Monday-Friday, 0800-1700.

Turn Around Time:

Final results within 1 week.

Reference Range:

Male:  X and Y probe signals          Female:  two X probe signals
For autosomes, the reference range varies for each probe (set).  
Suitable for interphase and metaphase analysis.  Call lab for 
information.

Comments:

This test can be used for screening of sex chromosomes, for monosomies in cases such as glioma (eg.1p, 5, 7,19q, etc.), for trisomies (eg., +4, +8, +11, +12, +13, +14, +18, +19, +21, +22, etc.) or for detection of the gene fusion resulting from Translocations (such as IgH/MYC, BCR/ABL, Igh/CCND1, IgH/BCL2) in solid tumors.

If the desired FISH probe is not listed, call the laboratory.

Shivanand R. Patil Cytogenetics & Molecular Laboratory Testing Menu

CPT Code:

88271 x # of probes
88275 x # of probes, 88291

See Additional Information:
Cytogenetics and Molecular Testing