Bordetella Pertussis Ab
Label Mnemonic: BORDPAN
Epic code: LAB4803
Downtime form: A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
Commercial Mail-out Laboratory
5231 RCP
356-8593
Specimen(s):
Serum
Specimen Instructions:
Parallel testing preferred. Mark specimen as acute or convalescent. Convalescent specimen must be received within 30 day from receipt of acute specimen. Severely lipemic specimen is not acceptable.
Collection Medium:
Red top tube 5 mL (Clot Activator)
Minimum:
Preferred minimum:  1 mL serum
Absolute minimum:  0.3 mL serum (does not allow for repeat)
Rejection Criteria:
Contaminated, heat-inactivated, or severely lipemic specimens.
Turn Around Time:
1-5 days upon receipt at reference laboratory
Reference Range:
Bordetella pertussis Antibody, IgG by ELISA with Reflex 
to Immunoblot
  Bordetella pertussis Antibody, IgG by ELISA
    0.94 IV OR LESS: Negative - No significant level of detectable
    B. pertussis IgG antibody.

    0.95-1.04 IV: Equivocal - Repeat testing in 10-14 days may be
    helpful.

    1.05 IV or greater: Positive - IgG antibody to B.
    pertussis detected, which may indicate a current or recent
    exposure/imunization to B. pertussis.

  Bordetella pertussis Antibody, IgG by Immunoblot
    Refer to report
______________________________________________________________________

Bordetella pertussis Antibody, IgM by ELISA with Reflex to 
Immunoblot
  Bordetella pertussis Antibody, IgM by ELISA
    0.9 IV or less: Negative - No significant level of detectable
    B. pertussis IgM antibody.

    1.0-1.1 IV: Equivocal - Repeat testing in 10-14 days may be
    helpful.

    1.2 IV or greater: Positive - IgM antibody to B. pertussis
    detected, which may indicate a current or recent exposure/
    immunization to B. pertussis. 

  Bordetella pertussis Antibody, IgM by Immunoblot
    Refer to report
Interpretive Data:
Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test. B. pertussis IgM test by ELISA may produce false-positive results.
Comments:
Note: If Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added; if Bordetella pertussis Antibody, IgM by ELISA is 1.2 IV or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
Methodology:
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
CPT Code:
86615 x2; if reflexed, add 86615 for each Immunoblot