Toll-Like Receptor Function
Label Mnemonic: TLR
Epic Lab Code: LAB4609
Downtime Form: A-1a Miscellaneous Request
Commercial Mail-out Laboratory
5231 RCP
356-8593
Specimen(s):
Whole Blood
Specimen Instructions:
Patient and control samples must be collected within 48 hours of test performance.

The control specimen needs to be drawn from a normal, healthy individual who is not biologically related to the patient, and drawn at approximately the same time as and under similar conditions to the patient specimen.
Collection Medium:
and plus control and
Yellow top tube 8.5 mL (ACD solution A) Yellow top tube 8.5 mL (ACD solution A) Yellow top tube 8.5 mL (ACD solution A) Yellow top tube 8.5 mL (ACD solution A)
Minimum:
Preferred Minimum: One 10 mL whole blood and one 10 mL normal control from a healthy non-related individual. Absolute Minimum: 7 mL whole blood and 7 mL normal control from a healthy non-related individual. Infant Minimum: 3 mL whole blood and 7 mL normal control from a healthy non-related individual.
Rejection Criteria:
Yellow (ACD Solution B). Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection.
Turn Around Time:
9-10 days upon receipt at reference laboratory
Reference Range:
By report
Interpretive Data:
Toll-like receptors (TLR), PAM3CSK4, synthetic bacterial lipoprotein (TLR2-TLR1 ligand); zymosan cell wall particles from Saccharomyces cerevisiae (TLR6-TLR2 ligand); poly(I:C), synthetic analog of dsRNA (TLR3 ligand); lipopolysaccharide ultra pure S. minnesota LPS (TLR4 ligand); flagellin purified from S. thyphimurium (TLR5 ligand); and CL097 imidazoquinoline compound (TLR7-TLR8 ligand) , are tested independently in peripheral blood mononuclear cell (PBMC) culture. PBMC cytokine production responses to these mitogens are determined by multi-analyte fluorescence detection.
Comments:
Results for TNF alpha, IL-1 beta, and IL-6 are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by the reference laboratory.

This mailout test requires pathologist approval for orders during inpatient encounters. Mailouts staff will not process order without approval. The pathologist covering mailouts approval can be reached at pager #5379. If approval is given, the name of the pathologist can be selected in the drop-down menu to the right of the approval warning in Epic when ordering the test.
Methodology:
Cell Culture/Quantitative Multiplex Bead Assay
CPT Code:
86353(x6), 83520(x3)