Herpes Virus 6 (HHV-6) Quant by PCR

Label Mnemonic:	HHV6PCR
Epic code:	LAB4619
Downtime form:	Doctor/Provider Orders - Pathology Core and Specialty Care Nursery

Commercial Mailout Laboratory
6240-8 RCP
356-8593

Specimen(s):

Plasma

Collection Medium:

Lavender top tube 3 mL (EDTA)

Minimum:

Preferred Minimum: 1 mL plasma
Absolute Minimum: 0.5 mL plasma

Rejection Criteria:

Heparinized samples.

Delivery Instructions:

Submit specimen to laboratory as soon as possible after collection.

Turn Around Time:

1-4 days upon receipt at reference laboratory

Reference Range:

Not detected.

Interpretive Data:

The quantitative range of this assay is 3.0-6.0 log copies/mL (1,000-999,000 copies/mL)

A negative result (less than 3.0 log copies/mL or less than 1,000 copies/mL) does not rule out the presence of PCR inhibitors in the patient specimen or HHV6 DNA in concentrations below the level of detection of the assay. Inhibition may also lead to underestimation of viral quantitation.

No international standard is currently available for calibration of this assay. Caution should be taken when interpreting results generated by different assay methodologies.

Comments:

Note: The limit of quantification for this DNA assay is 3.0 log copies/mL (1,000 copies/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<3.0 log copies/mL (<1,000 copies/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified".

This assay detects and quantifies HHV6 subtypes A and B.

Methodology:

Quantitative Polymerase Chain Reaction

CPT Code:

87533