Quantitative STR CD3 Subset Analysis (VAMC)

Iowa Regional Histocompatibility and Immunogenetics
Iowa City Veterans Affairs Medical Center 10E-19
319-338-0581, EXT. 5640 (dial 158 from UIHC)

Specimen(s):

Whole Blood

Minimum:

THREE - FOUR 10 mL yellow top (ACD) tubes.

Delivery Instructions:

Deliver at room temperature. Sample must arrive by 11:00 a.m.

Testing Schedule:

Test performed daily.

Turn Around Time:

Results in Epic by 5-7 working days.

Comments:

Requires pre-transplant donor sample AND pre-transplant recipient 
sample and prior Qualitative STR testing.

Engraftment test provides percentage of donor DNA in purified CD3 
subset from post-transplant recipient blood sample by quantitative 
analysis of 1 STR locus.

All HLA Testing is ordered through the University of Iowa Epic 
System.

Methodology:

Polymerase Chain Reaction (PCR), magnetic cell separation, flow cytometry and Sequence Based Typing (SBT).

CPT Code:

81265, 81266, 81268, 86849, 88184, 88185(x2), 88187

See Additional Information:
Iowa Regional Histocompatibility and Immunogenetics Laboratory Required Content on Requisitions