Quantitative STR CD3 Subset Analysis (VAMC)
Iowa Regional Histocompatibility and Immunogenetics
Veterans Affairs Hospital 10E-19
319-338-0581, EXT. 5640
(dial 158 from UIHC)
Specimen(s):
Whole Blood
Minimum:
THREE - FOUR 10 mL yellow top (ACD) tubes.
Delivery Instructions:
Deliver at room temperature. Sample must arrive by 11:00 a.m.
Testing Schedule:
Test performed daily.
Turn Around Time:
Results in Epic by 5-7 working days.
Comments:
Requires pre-transplant donor sample AND pre-transplant recipient sample and prior Qualitative STR testing. Engraftment test provides percentage of donor DNA in purified CD3 subset from post-transplant recipient blood sample by quantitative analysis of 1 STR locus. All HLA Testing is ordered through the University of Iowa Epic System.
Methodology:
Polymerase Chain Reaction (PCR), magnetic cell separation, flow cytometry and Sequence Based Typing (SBT).
CPT Code:
81265, 81266, 81268, 86849, 88184, 88185(x2), 88187