BK Virus DNA Quant, PCR
Label Mnemonic: BKQNTP
Epic Lab Code: LAB2722
Downtime Form: A-1a Miscellaneous Request
Commercial Mail-out Laboratory
5231 RCP
356-8593
Specimen(s):
Plasma
Collection Medium:
Lavender top tube 3 mL (EDTA)
Minimum:
Preferred Minimum: 1 mL plasma in a lavender top tube
Absolute Minimum: 0.5 mL plasma in a lavender top tube
Rejection Criteria:
Urine, frozen whole blood. Heparinized specimens.
Testing Schedule:
Reference Lab receives samples Monday-Saturday; Mailouts ships Monday- Friday.
Turn Around Time:
1-3 days upon receipt at reference laboratory
Reference Range:
Not detected
Interpretive Data:
The quantitative range of this assay is 2.6-8.6 log copies/mL (390- 390,000,000 copies/mL).

A negative result (less than 2.6 log copies/mL or less than 390 copies/mL) does not rule out the presence of PCR inhibitors in the patient specimen or BK virus DNA concentrations below the level of detection of the assay. Inhibition may also lead to underestimation of viral quantitation.

No international standard is currently available for calibration of this assay. Caution should be taken when interpreting results generated by different assay methodologies.
Comments:
The limit of quantification for this DNA assay is 2.6 log copies/mL (390 copies/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "< 2.6 log copies/mL (< 390 copies/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified."
Methodology:
Quantitative Real-Time Polymerase Chain Reaction
CPT Code:
87799