Serum

Draw TWO red top tubes
Preferred Minimum: 5 mL serum
Absolute Minimum: 4 mL serum (Does not allow for repeat testing)

16 days upon receipt at reference laboratory

Anti-PL-7 Ab, Anti-PL-12 Ab, Anti-EJ Ab, Anti-OJ Ab, Anti-SRP Ab, Anti-Mi-2-Ab, Anti-U3 RNP (Fibrillarin), Anti-U2 RNP Ab, Anti-Ku Ab: Negative

Interpretation for: Anti-Jo-1 Ab, Anti-TIF-1gamma-Ab, Anti-MDA-5-Ab (CADM-140), Anti-NXP-2 (P140) Ab, Anti-SAE1 Ab IgG, Anti-PM/Scl-100 Ab, Anti-SS-A 52kD Ab IgG, Anti-U1-RNP Ab:

Reference Range: <20
Negative: <20 units
Weak Positive: 20 - 39 units
Moderate Positive: 40 - 80 units

Anti-Jo 1 Abs are found in subset of myositis patients characterized by interstitial lung disease, systemic polyarthritis, Raynaud's Phenomena, fever and Mechanic's Hand (anti-synthetase syndrome).

Anti-Jo 1 appears to be a marker for interstitial lung disease in polymyositis.

This mailout test requires pathologist approval for orders during inpatient encounters. Mailouts staff will not process order without approval. The pathologist covering mailouts approval can be reached at pager #3724. If approval is given, the name of the pathologist can be selected in the drop-down menu to the right of the approval warning in Epic when ordering the test.

Useful for: The MyoMarker Panel 3 Plus can be used to assist in the diagnosis of dermatomyositis, polymyositis and the anti-synthetase syndrome. Furthermore, it allows characterization of various subsets of these disorders and offers prognostic information.

Enzyme-linked immunosorbent assay (ELISA); RIPA Gel Radiography

83516 x 9 - PL-7, PL12, EJ, OJ, U2 snRNP, Anti-Fibrillarin U3 RNP, Mi-2, SRP, Ku

86235 x 8 - Anti-Jo-1-Ab, Anti-SSA 52 kD Ab, IgG, Anti-PM/Scl Ab, Anti-U1 RNP Ab,MDA-5(P140) (CADM), NXP-2 (P140), TIF1 GAMMA (P155/140), Anti-SAE 1 IgG