JAK2 Exons 12, 13, and 14 including V617F Mutation Analysis with Interpretation

Label Mnemonic:	JAK2
Epic code:	LAB2472
Downtime form:	Doctor/Provider Orders - Pathology Molecular

Molecular Pathology
6240 RCP
384-9568

Specimen(s):

Whole Blood

Collection Medium:

Pink top tube 6 mL (K2-EDTA)

Minimum:

Adults 3 mL whole blood. Testing on smaller volumes than those requested will be attempted. However, in some cases, small blood volumes may compromise the ability to perform testing. Testing requires a dedicated collection tube.

Delivery Instructions:

Room temperature for up to 24 hours, then refrigerate the whole blood if it is necessary to be held overnight, weekends or holidays.

Testing Schedule:

Weekly

Turn Around Time:

7-10 working days upon receipt

Reference Range:

Direct detection of G-T transversion at position 1849 of the Janus kinase 2 locus, giving rise to a Valine-to-Phenylalanine substitution at position 617 (JAK2 V617F mutation).

Interpretive Data:

Refer to report

Comments:

This test is useful for diagnosis of myeloproliferative disorders. Note: Exon 14 includes the common V617F variant.

Methodology:

Massively parallel next generation DNA sequencing by synthesis, using semiconductor technology.

CPT Code:

81270