Beta D Glucan (Fungitell)
Label Mnemonic: BDGLUCAN
Epic Lab Code: LAB2703
Downtime Form: A-1a Miscellaneous Request
Commercial Mail-out Laboratory
5231 RCP
356-8593
Specimen(s):
Serum
Specimen Instructions:
This test needs its own dedicated tube and cannot be shared with any other tests.
Collection Medium:
BD Gold SST 5 mL Vacutainer
Minimum:
Adult Absolute Minimum: 0.5 mL serum
Pediatric Absolute Minimum: 0.2 mL serum
Rejection Criteria:
Lipemic, icteric, or hemolyzed specimens. Specimens that have been stored at ambient temperature. Specimens that have been stored at 2-8°C for >5 days. If storage longer than 5 days is needed, samples should be frozen at -70°C. Unless indicated as stored frozen, the specimen will be rejected if the draw date is >5 days from receipt at reference laboratory.
Turn Around Time:
24 hours upon receipt at reference laboratory
Reference Range:
Less than 60 pg/mL
Interpretive Data:
Glucan values of less than 60 pg/mL are interpreted as negative. Glucan values of 60 to 79 pg/mL are interpreted as indeterminate, and suggest a possible fungal infection. Additional sampling and testing of sera is required to interpret the results.

Glucan values of greater than or equal to 80 pg/mL are interpreted as positive. Due to the potential for environmental contamination when transferred to pour-off tubes, which can lead to false positive results, interpret positive results from samples provided in pour-off tubes with caution. Results should be used in conjunction with clinical findings, and should not form the sole basis for a diagnosis or treatment decision. Fungitell is FDA approved or cleared for in vitro diagnostic use.

The Fungitell assay does not detect certain fungal species such as the genus Cryptococcus (Tanaka et. al. 1991) which produces very low levels of (1-3)-Beta-D-Glucan. The assay also does not detect the Zygomycetes such as Absidia, Mucor and Rhizopus (Mitsuya et al. 1994) which are not known to produce (1-3)-Beta-D-Glucan. In addition, the yeast phase of Blastomyces dermatitidis produces little (1-3)-Beta-D-Glucan and may not be detected by the assay (Girouard et al. 2007).
Methodology:
Enzyme Immunoassay (EIA)
CPT Code:
87449