Formalin fixed, paraffin embedded tissue block or 10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue

Tumor cells more than 50% of the total tissue and greater than 10mm² in surface area on the block.

Must also submit a healthy tissue specimen or peripheral blood from the same subject for comparison of microsatellite markers.

Specimens fixed in B5 fixative or that have been decalcified will not be accepted. Tumor specimens containing less than 50% tumor cells or are less than 10mm² in area may be unacceptable.

Weekly

7-10 working days

Microsatellite Stable

Tumor tissue and normal tissue should be obtained as part of the surgical procedure for testing. The tissue should be formalin-fixed, paraffin embedded and reviewed by a surgical pathologist prior to MSI testing. A block containing normal tissue (non-tumor) should be submitted for testing in addition to a block containing at least 50% tumor. Acceptable tissue for the non-tumor block is tissue with a high nuclear content, preferably benign lymph node tissue if available. Blood submitted in EDTA or sodium citrate is acceptable for the normal block if tissue is not available. An H&E stained glass slide from both blocks should also be submitted.

Multiplex PCR followed by Fluorescence Capillary Electrophoresis

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