TRG Gene Clonality by PCR with Interpretation
Label Mnemonic: TCELLPCR
Epic Lab Code: LAB2487 for Tissue
LAB7360 for Blood
Downtime Form: A-1a Miscellaneous Request
Molecular Pathology
6004 BT GH
384-9568
Collection Medium:
and or
Lavender top tube 3 mL (EDTA) Lavender top tube 3 mL (EDTA) Yellow top tube 8.5 mL (ACD solution A)
Minimum:
5 mL blood in a lavender top or yellow top, 1 mL bone marrow aspirate in a lavender top or yellow top, 3 cu mm of fresh frozen tissue or Formalin-fixed, paraffin embedded tissue, body fluids in a lavender top or yellow top or lymph node aspirates in RPMI.
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection.
Testing Schedule:
Weekly
Turn Around Time:
7 working days
Reference Range:
None
Comments:
DNA extracted from bone marrow mononuclear cells or tissue is examined for rearrangement of T-cell receptor gamma (TCRG) genes.
Test Limitations:
Specimens fixed in B5 fixative are unsuitable for testing.
Methodology:
Multiplex Polymerase Chain Reaction (PCR) followed by Fluorescence Capillary Electrophoresis
CPT Code:
81342