IGH Gene Clonality by PCR with Interpretation

Label Mnemonic:	IGH, IGHGENE
Epic code:	LAB8283 for Blood, LAB2471 for Bone Marrow and Tissue
Downtime form:	A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery

Molecular Pathology
6240 RCP
384-9568

Specimen(s):

Blood, Bone Marrow and Tissue

Collection Medium:

	or
Pink top tube 6 mL (K2-EDTA)	or	Yellow top tube 8.5 mL (ACD solution A)

Minimum:

5 mL blood in a pink top or yellow top, 1 mL bone marrow aspirate in a pink top or yellow top, 3 cu mm of fresh frozen tissue or Formalin-fixed, paraffin embedded tissue, body fluids in a pink top or yellow top or lymph node aspirates in RPMI.

Delivery Instructions:

Submit specimen to laboratory as soon as possible after collection.

Testing Schedule:

Weekly

Turn Around Time:

7 working days

Reference Range:

None

Comments:

DNA extracted from blood, bone marrow mononuclear cells or tissue is examined for rearrangement of immunoglobin heavy chain genes.

NOTE: For patients with chronic lymphocytic leukemia, if analysis for IGH gene somatic hypermutation (or IGH variable region mutation) is desired, please order LAB7503 or LAB7504.

Test Limitations:

Specimens fixed in B5 fixative are unsuitable for testing.

Methodology:

Multiplex PCR followed by Fluorescence Capillary Electrophoresis

CPT Code:

81261