IGH Gene Clonality by PCR with Interpretation
Label Mnemonic: BCELLPCR
Epic Lab Code: LAB2471 for Tissue
LAB8283 for Blood
Downtime Form: A-1a Miscellaneous Request
Molecular Pathology
6004 BT GH
384-9568
Specimen(s):
Various
Collection Medium:
or
Pink top tube 6 mL (K2-EDTA) Yellow top tube 8.5 mL (ACD solution A)
Minimum:
5 mL blood in a pink top or yellow top, 1 mL bone marrow aspirate in a pink top or yellow top, 3 cu mm of fresh frozen tissue or Formalin-fixed, paraffin embedded tissue, body fluids in a pink top or yellow top or lymph node aspirates in RPMI.
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection.
Testing Schedule:
Weekly
Turn Around Time:
7 working days
Reference Range:
None
Comments:
DNA extracted from blood, bone marrow mononuclear cells or tissue is examined for rearrangement of immunoglobin heavy chain genes.
Test Limitations:
Specimens fixed in B5 fixative are unsuitable for testing.
Methodology:
Multiplex PCR followed by Fluorescence Capillary Electrophoresis
CPT Code:
81261