Fluorescence In-Situ Hybridization (FISH-Bone Marrow)
Epic Lab Code: CYT54
Downtime Form: C-12 Cytogenetics Request
Shivanand R. Patil Cytogenetics & Molecular Laboratory
Department of Pediatrics
W-101 GH
356-3877 (Laboratory)
Bone Marrow, Hematologic Malignancies, Lymph Node, Fine-Needle Aspirate, Pleural Effusion, CSF
Specimen Instructions:
Provide details of clinical information. If a specimen is collected over the weekend, please page the technologist on call by dialing 1-888-533-0186. When it stops ringing, enter your phone number, the '#' sign, and hang up.
Marrow Aspirate: 1-2 cc of bone marrow collected in a bone marrow media tube available from our lab or in a green-top Vacutainer® with sodium heparin. Invert tube to mix well. Label the tube with patient name and medical record number. DO NOT FREEZE OR CENTRIFUGE.

Lymph Node: piece of lymph node biopsy

Tumor Fluid: collect between 1-5cc fluid with good cellularity
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection.
Testing Schedule:
Specimens accepted in the lab Monday-Friday, 0800-1700. After hours specimens should be taken to specimen control and a message left on the lab voice mail. In the case of an emergency, follow the instructions on the lab voice mail.
Turn Around Time:
Allow 3-5 days for final results. Contact laboratory if STAT TAT required.
Reference Range:
Varies for each probe (set). Unique sequence probes labeled with fluorochrome. Contact lab for details.
This test can be used as an adjunct to chromosomal analysis for detection of clonal abnormalities including residual disease. Suitable for interphase and metaphase analysis.

Probes currently available include:
ALK [2p23]
AML1/ETO [t(8;21)]
BCR/ABL [t(9;22)]
CBFB [inv (16)]
Cep7/D7S522[-7 and del(7q31)]*
Cep8 [trisomy 8]
Cep12 [trisomy 12]
CLL probe panel
ETV6/RUNX1 [t(12;21)]
MLL [del(11)(q23)]
N-MYC [2p24.1] or for amplification 2p24.1]
PML/RARA [t(15;17)]
Rb1 [del(13)(q14)]
If the desired FISH probe is not listed, call the laboratory.

*CEP = centromere enumeration probe

Shivanand R. Patil Cytogenetics & Molecular Laboratory Website
Fluorescence In-Situ Hybridization (FISH)
CPT Code:
88271 x # of probes
88275 x # of probes, 88291
See Additional Information:
Cytogenetics and Molecular Testing