Fluorescence In-Situ Hybridization (FISH-Hematological Blood)
Epic Lab Code: CYT54
Downtime Form: C-12 Cytogenetics Request
Shivanand R. Patil Cytogenetics & Molecular Laboratory (Dept. of Pediatrics)
W-101 GH
356-3877 (Laboratory)
Specimen(s):
Peripheral Blood
Specimen Instructions:
Provide details of clinical information. If a specimen is collected over the weekend, please page the technologist on call by dialing 1-888-533-0186. When it stops ringing, enter your phone number, the '#' sign, and hang up.
Collection Medium:
Green top tube 10 mL (Na Heparin) Hospital Stores #907693
Minimum:
5-10 cc adult, 2 cc infants of venous blood collected in a green-top Vacutainer® with sodium heparin. Invert tube to mix well. Label the tube with patient name and medical record number. DO NOT FREEZE OR CENTRIFUGE.
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection.
Testing Schedule:
Specimens accepted in the lab Monday-Friday, 0800-1700. After hours specimens should be taken to specimen control and a message left on the lab voice mail. In the case of an emergency, follow the instructions on the lab voice mail.
Turn Around Time:
Allow 3-5 days for final results.
Reference Range:
Varies for each probe (set). Unique sequence probes labeled with fluorochrome. Contact lab for details.
Comments:
This test can be used as an adjunct to chromosomal analysis for detection of residual disease. Suitable for interphase and metaphase analysis.

Probes currently available include:
ALK [2p23]
AML1/ETO [t(8;21)]
BCR/ABL [t(9;22)]
CBFB [inv (16)]
Cep7/D7S522[-7 and del(7q31)]
Cep8 [trisomy 8]
Cep12 [trisomy 12]
CepX/Y[transplants]
CLL probe panel
D13S319[del(13q)]
D13S25[del(13q)]
EGR1[del(5q31)]
IgH/BCL-2[t(14;18)]
IgH/CCND1[t(11;14)]
IgH/MYC[t(8;14)]
MLL [del(11)(q23)]
N-MYC [2p24.1 or for amplification 2p24.1]
p53 [del(17)(p13.1)]
PML/RARA [t(15;17)]
Rb1 [del(13)(q14)]
TEL/AML1 [t(12;21)]

If the desired FISH probe is not listed, call the laboratory.

*CEP = centromere enumeration probe

Shivanand R. Patil Cytogenetics & Molecular Laboratory Website
Methodology:
Fluorescence In-Situ Hybridization (FISH)
CPT Code:
88271 x # of probes
88275 x # of probes, 88291
 
See Additional Information:
Cytogenetics and Molecular Testing