Thyroid Stimulating Hormone (TSH)
| Label Mnemonic: | TSH |
| Epic code: | LAB129 |
| Downtime form: | A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery |
Chemistry
6240 RCP
356-3527
6240 RCP
356-3527
Specimen(s):
Plasma
Collection Medium:
 |
| Plasma Separator Tube 4.5 mL |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
4 mL whole blood in light green top or TWO
Microtainer® devices.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
2 hours (upon receipt in laboratory)
Reference Range:
AGE MALES AND FEMALES 0 - 11 months 0.8-6.3 μIU/mL 1 - 5 years 0.7-5.9 μIU/mL > 5 years Same as adult values Adults 0.27-4.20 μIU/mL
Comments:
Refer to BD Microtainer® Tubes product sheet for detailed sample collection
instructions.
Samples which are part of a TRH stimulation test should be clearly identified as such.
This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).
Samples which are part of a TRH stimulation test should be clearly identified as such.
This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).
Test
Limitations:
The assay is unaffected by icterus (bilirubin is less than 41
mg/dL), hemolysis (Hb is less than 1000 mg/dL), lipemia (Intralipid is
less than 1500 mg/dL) and biotin is less than 1200 ng/mL (criterion:
recovery within plus or minus 10% of initial value).
This TSH assay is not significantly impacted by high-dose biotin. Studies have shown that serum concentrations of biotin did not exceed 1200 ng/mL in subjects taking a single dose of 300 mg biotin.
No interference was observed from rheumatoid factor (up to 1500 U/mL) and samples from dialysis patients.
There is no high-dose hook effect for TSH concentrations up to 1000 μIU/mL.
In vitro tests were performed on 17 commonly used pharmaceuticals. No interference with the assay was found.
The presence of autoantibodies may induce high molecular weight complexes (macro-TSH) which may cause unexpectedly high values of TSH.
As with all tests containing monoclonal mouse antibodies, erroneous findings may be obtained from samples taken from patients who have been treated with monoclonal mouse antibodies or have received them for diagnostic purposes.
In rare cases, interference due to extremely high titers of antibodies to ruthenium can occur. TSH reagent contains additives which minimize these effects.
Extremely high titers of antibodies to streptavidin can occur in isolated cases and cause interference.
For diagnostic purposes, the TSH findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
This TSH assay is not significantly impacted by high-dose biotin. Studies have shown that serum concentrations of biotin did not exceed 1200 ng/mL in subjects taking a single dose of 300 mg biotin.
No interference was observed from rheumatoid factor (up to 1500 U/mL) and samples from dialysis patients.
There is no high-dose hook effect for TSH concentrations up to 1000 μIU/mL.
In vitro tests were performed on 17 commonly used pharmaceuticals. No interference with the assay was found.
The presence of autoantibodies may induce high molecular weight complexes (macro-TSH) which may cause unexpectedly high values of TSH.
As with all tests containing monoclonal mouse antibodies, erroneous findings may be obtained from samples taken from patients who have been treated with monoclonal mouse antibodies or have received them for diagnostic purposes.
In rare cases, interference due to extremely high titers of antibodies to ruthenium can occur. TSH reagent contains additives which minimize these effects.
Extremely high titers of antibodies to streptavidin can occur in isolated cases and cause interference.
For diagnostic purposes, the TSH findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
Methodology:
Electrochemiluminescence Immunoassay
Assay updated with increased tolerance to biotin on 08-25-2020.
Assay updated with increased tolerance to biotin on 08-25-2020.
CPT Code:
84443
See also:
Thyroid Stimulating Hormone (TSH), Reflexive, Plasma
Thyroid Stimulating Hormone (TSH), Reflexive, Plasma