Progesterone

Label Mnemonic:	PRGS
Epic code:	LAB529
Downtime form:	Doctor/Provider Orders - Pathology Core and Specialty Care Nursery

Chemistry
6240 RCP
356-3527

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL whole blood in light green top tube or TWO Microtainer^® devices.

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

Females:
  Adult females:
    Follicular phase:      0.2 - 1.5 ng/mL
    Ovulation phase:       0.8 - 3.0 ng/mL
    Luteal phase:          1.7 - 27.0 ng/mL
    Post-menopausal:       < 0.2 - 0.8 ng/mL
  Pediatric females:
    < 2 years old:         0.87 - 3.37 ng/mL
    2-9 years old:         0.20 - 0.24 ng/mL
    10-17 years old:       adult levels generally achieved by puberty

Males:
  Adult males:             0.2 - 1.4 ng/mL
    < 2 years old          0.87 - 3.37 ng/mL
    2-9 years old          < 0.2 ng/mL
    10-17 years old        adult levels generally achieved by puberty

Reference: Lippe BM, LaFranchi SH, Lavin N, et al: Serum 17-alpha-
hydroxyprogesterone, progesterone, estradiol, and testosterone in the 
diagnosis and management of congenital adrenal hyperplasia. J Pediatr 
1974;85:782-787.

Comments:

This assay may be significantly impacted by high-dose biotin (>5 mg dose) taken within previous 12 hours. High concentrations of biotin may lead to falsely increased results. These concentrations may be found in patients taking over-the-counter supplements with biotin content much higher than nutritional requirements for biotin. Specimens should not be collected until at least 12 hours after the last dose.

Test Limitations:

The assay is unaffected by icterus = 54(bilirubin ditaurate is 
less than 54 mg/dL), hemolysis = 1000 (Hb is less than 1.0 g/dL), 
lipemia = 200 (Intralipid is less than 720 mg/dL) and biotin is less 
than 20 ng/mL. (criterion: recovery within plus or minus 10% of 
initial value).

No interference was observed from rheumatoid factors up to a 
concentration of 2000 IU/mL.

In vitro tests were performed on 18 commonly used pharmaceuticals. Of 
these, only phenylbutazone at therapeutic dosage levels showed 
interference with the assay (progesterone values depressed).

As with all tests containing monoclonal antibodies, erroneous findings 
may be obtained from samples taken from patients who have been treated 
with monoclonal mouse antibodies or who have received them for 
diagnostic purposes.

In rare cases, interference due to extremely high titers of antibodies 
to ruthenium can occur.

Progesterone II contains additives which minimize these effects.

Extremely high titers of antibodies to streptavidin can occur in 
isolated cases and cause interference.

For diagnostic purposes, the Progesterone II findings should always be 
assessed in conjunction with the patient's medical history, clinical 
examination and other findings.

Methodology:

Electrochemiluminescence Immunoassay

CPT Code:

84144

See Additional Information:
Biotin Interference with Immunoassays