Myoglobin
Label Mnemonic: MYO
Epic Lab Code: LAB105
Downtime Form: A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
Chemistry
6240 RCP
356-3527
Specimen(s):
Plasma
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top tube or TWO Microtainer® devices
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
Males: 28-72 ng/mL Females: 25-58 ng/mL
Comments:
This assay may be significantly impacted by high-dose biotin (>5 mg dose) taken within previous 12 hours. High concentrations of biotin may lead to falsely decreased results. These concentrations may be found in patients taking over-the-counter supplements with biotin content much higher than nutritional requirements for biotin. Specimens should not be collected until at least 12 hours after the last dose.
Test Limitations:
The assay is unaffected by icterus (bilirubin is less than 65 mg/dL), hemolysis (Hb is less than 1400 mg/dL), lipemia (less than 2200) and biotin is less than 50 ng/mL. No interference was observed from rheumatoid factor up to 1500 U/mL. There is no high-dose hook affect for myoglobin concentrations up to 30,000 mg/mL. In vitro tests were performed on 50 commonly used pharmaceuticals. No interference with the assay was found. As with all tests containing monoclonal mouse antibodies, erroneous findings may be obtained from samples taken from patients who have been treated with monoclonal mouse antibodies or have received them for diagnostic purposes. In rare cases, interference due to extremely high titers of antibodies to Ruthenium can occur. The Roche myoglobin reagent contains additives which minimize these effects. Extremely high titers of antibodies to Streptavidin can occur in isolated cases and cause interference. For diagnostic purposes, the Myoglobin findings should always be assessed in conjunction with their patient's medical history, clinical examination and other findings.
Methodology:
Electrochemiluminescence Immunoassay
CPT Code:
83874
 
See Additional Information:
Biotin Interference with Immunoassays