H. influenza IgG Ab.

Label Mnemonic:	HFLU
Epic code:	LAB3257
Downtime form:	Doctor/Provider Orders - Pathology Core and Specialty Care Nursery

Commercial Mailout Laboratory
6240-8 RCP
356-8593

Specimen(s):

Serum

Specimen Instructions:

Collect "Pre" and 30-day "post" Haemophilus influenzae b vaccination specimens should be submitted together for testing. "Post" specimen should be drawn 30 days after immunization and must be received within 60 days of "pre" specimen.

Collection Medium:

Red top tube 5 mL (Clot Activator)

Minimum:

Preferred Minimum: 1 mL serum
Absolute Minimum: 0.15 mL serum
Mark specimens clearly as "Pre-Vaccine" or "Post- Vaccine".

Rejection Criteria:

Plasma or other body fluids. Contaminated, hemolyzed or, severely lipemic specimens.

Turn Around Time:

1-2 days upon receipt at reference laboratory

Reference Range:

< 1.0 μg/mL = Antibody concentration not protective.
≥ 1.0 μg/mL = Antibody to H. influenzae b detected. Suggestive of

              protection.

Interpretive Data:

Responder status is determined according to the ratio of post- vaccination concentration to pre-vaccination concentration of Haemophilus influenza b antibody, IgG as follows:
1. If the post-vaccination concentration is less than 3.0 μg/mL, the patient is considered to be a nonresponder.

2. If the post-vaccination concentration is greater than or equal to 3.0 μg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a nonresponder.

Methodology:

Quantitative Multiplex Bead Assay

CPT Code:

86317