H. influenza IgG Ab.
Label Mnemonic: HFLU
Epic Lab Code: LAB3257
Downtime Form: A-1a Miscellaneous Request
Commercial Mail-out Laboratory
5231 RCP
356-8593
Specimen(s):
Serum
Specimen Instructions:
Collect "Pre" and 30-day "post" Haemophilus influenzae b vaccination specimens should be submitted together for testing. "Post" specimen should be drawn 30 days after immunization and must be received within 60 days of "pre" specimen.
Collection Medium:
Red top tube 5 mL (Clot Activator)
Minimum:
Preferred Minimum: 1 mL serum
Absolute Minimum: 0.15 mL serum
Mark specimens clearly as "Pre-Vaccine" or "Post- Vaccine".
Rejection Criteria:
Plasma or other body fluids. Contaminated, hemolyzed or, severely lipemic specimens.
Turn Around Time:
1-2 days upon receipt at reference laboratory
Reference Range:
< 1.0 μg/mL = Antibody concentration not protective.
> 1.0 μg/mL = Antibody to H. influenzae b detected. Suggestive of
              protection.
Interpretive Data:
Responder status is determined according to the ratio of post- vaccination concentration to pre-vaccination concentration of Haemophilus influenza b antibody, IgG as follows:
1. If the post-vaccination concentration is less than 3.0 μg/mL, the patient is considered to be a nonresponder.

2. If the post-vaccination concentration is greater than or equal to 3.0 μg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a nonresponder.
Methodology:
Quantitative Multiplex Bead Assay
CPT Code:
86317