Amikacin Drug Level
|Plasma Separator Tube 4.5 mL
Call laboratory for additional acceptable specimen collection containers.
3 mL whole blood in light green top or ONE
Microtainer® for pediatric patients.
24 hrs/day, 7 days a week, including holidays.
1 hour (upon receipt in laboratory)
Peak levels: 20-25 μg/mL (45-75 minutes after I.M. dose or
15-30 minutes after I.V. dose).
Trough levels: 5-10 μg/mL (not more than 30 minutes before next
Critical value: >35 μg/mL
Kanamycin interferes with measurement of amikacin, giving falsely
Patient samples which contain kanamycin A, kanamycin B, 3', or 4'
dideoxykanamycin B will yield falsely elevated values for amikacin.
However, these drugs are not usually coadministered with amikacin.
High concentration of penicillins or cephalosporins have been shown to
inactivate aminoglycosides in vitro. The degree of inactivation is
dependent on the particular aminoglycoside being measured, the type
concentration of the penicillin or cephalosporin that is also present
and the storage conditions of the sample. Samples from patients
receiving additional antibiotics of these types should be assayed
immediately or stored frozen.
Hemolysis: No significant interference up to an H index of 1000
(approximate hemoglobin concentration: 1000 mg/dL).
Lipemia: No significant interference up to an L index of 2000
(approximate triglycerides concentration: 4000 mg/dL). Note there is
poor correlation between turbidity and triglyceride concentration.
Icterus: No significant interference up to an I index of 50
(approximate conjugated and unconjugated bilirubin concentration: 50
Kinetic Interaction of Microparticles in Solution (KIMS)