Amikacin Drug Level
Label Mnemonic: AMIK
Epic Lab Code: LAB555
Downtime Form: A-1a Therapeutic Drug Assay Requisition
Chemistry
6240 RCP
356-3527
Specimen(s):
Plasma
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top or ONE Microtainer® for pediatric patients.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
Peak levels: 20-25 μg/mL (45-75 minutes after I.M. dose or 15-30 minutes after I.V. dose). Trough levels: 5-10 μg/mL (not more than 30 minutes before next dose). Critical value: >35 μg/mL
Comments:
Kanamycin interferes with measurement of amikacin, giving falsely elevated results.
Test Limitations:
Patient samples which contain kanamycin A, kanamycin B, 3', or 4' dideoxykanamycin B will yield falsely elevated values for amikacin. However, these drugs are not usually coadministered with amikacin. High concentration of penicillins or cephalosporins have been shown to inactivate aminoglycosides in vitro. The degree of inactivation is dependent on the particular aminoglycoside being measured, the type and concentration of the penicillin or cephalosporin that is also present and the storage conditions of the sample. Samples from patients receiving additional antibiotics of these types should be assayed immediately or stored frozen.

Hemolysis: No significant interference up to an H index of 1000 (approximate hemoglobin concentration: 1000 mg/dL).

Lipemia: No significant interference up to an L index of 2000 (approximate triglycerides concentration: 4000 mg/dL). Note there is poor correlation between turbidity and triglyceride concentration.

Icterus: No significant interference up to an I index of 50 (approximate conjugated and unconjugated bilirubin concentration: 50 mg/dL).
Methodology:
Kinetic Interaction of Microparticles in Solution (KIMS)
CPT Code:
80150
 
See Additional Information:
Chemistry Critical Lab Values