Immunoglobulin E (IgE), Individual Quant
Label Mnemonic: IGE
Epic Lab Code: LAB74
Downtime Form: A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
6240 RCP
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
3 mL whole blood from light green top tube or TWO Microtainer® devices
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
Non-allergic Adult: 0-100 IU/mL Non-allergic Children: 10-20% of adult values Neonates: 0.0-1.5 IU/mL Infants in 1st year of life: 0-15 IU/mL Children aged 1-5 years: 0-60 IU/mL Children aged 6-9 years: 0-90 IU/mL Children aged 10-15 years: 0-200 IU/mL
This assay may be significantly impacted by high-dose biotin (>5 mg dose) taken within previous 12 hours. High concentrations of biotin may lead to falsely decreased results. These concentrations may be found in patients taking over-the-counter supplements with biotin content much higher than nutritional requirements for biotin. Specimens should not be collected until at least 12 hours after the last dose.
Test Limitations:
The assay is unaffected by icterus (bilirubin is less than 37 mg/dL), hemolysis (Hb is less than 100 mg/dL), lipemia (triglycerides is less than 2200 mg/dL) and biotin is less than 100 ng/mL. (criterion: recovery within plus or minus 10% of initial value). No interference was observed from rheumatoid factor up to 6000 U/mL (method comparison: Elecsys IgE and a comparison IgE test on 50 samples). No high dose hook effect at IgE concentrations of up to 50 000 IU/mL (120000 ng/mL). In vitro tests were performed on 36 commonly used pharmaceuticals. No interference with the assay was found. As with all tests containing monoclonal mouse antibodies, erroneous findings may be obtained from samples taken from patients who have been treated with monoclonal mouse antibodies or have received them for diagnostic purposes. In rare cases interference due to extremely high titers of antibodies to ruthenium can occur. IgE contains additives which minimize these effects. Extremely high titers of antibodies to streptavidin can occur in isolated cases and cause interference. For diagnostic purposes, the IgE findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
Electrochemiluminescence Immunoassay
CPT Code:
See Additional Information:
Biotin Interference with Immunoassays