DNA Extraction, Cytogenetics
| Epic code: | CYT59 |
| Downtime form: | Cytogenetics Request |
Department of Pediatrics
W-101 GH
356-3877 (Laboratory)
 | and | ) |
| Lavender top tube 3 mL (EDTA) | Sterile Container |
Peripheral Blood:
For infants under 1 year of age: 1–2 mL in a lavender (EDTA)
For children over 1 year of age: 3–5 mL in a lavender (EDTA)
For adults: 5–7 mL in a lavender (EDTA)
Do not freeze blood specimens. Store at 4°C prior to delivery to the lab. Label all tubes with the patient name and medical record number.
Tissue:
Specimen obtained aseptically according to your protocol. Do not put specimen in alcohol or formalin. Specimen may be frozen prior to delivery. Label all containers with the patient name and medical record number.
Saliva & Buccal Swabs:
Contact the laboratory to obtain saliva or buccal swab collection kits and further instructions.
Submit specimen to laboratory as soon as possible after collection. Do not freeze.
After hours specimens should be taken to Specimen Control. For questions after hours, call (319) 356-1616 and ask the operator to page the cytogenetics on-call staff at pager #5525.
Specimens accepted in the lab Monday–Friday, 0800–1700.
Final results within 5 days
A brief interpretive report will be issued upon completion.
DNA is extracted following the Qiagen Gentra Puregene protocol, which produces high molecular weight genomic DNA. The quality of the DNA is verified by spectrophotometric analysis and/or fluorometric analysis prior to storage at -20°C.
The DNA will be held, however no testing will be performed unless further orders are received. Please contact the Cytogenetics and Molecular Laboratory with any further instructions or questions.
Shivanand R. Patil Cytogenetics & Molecular Laboratory Website