NPM1 Mutation Quantitative, MRD Monitoring and Interpretation

Label Mnemonic:	NPM1BMQNT
Epic code:	LAB8829
Downtime form:	Doctor/Provider Orders - Pathology Molecular

Molecular Pathology
6240 RCP
384-9568

Specimen(s):

Bone Marrow Aspirate

Specimen Instructions:

Testing requires dedicated collection tube.
DO NOT CENTRIFUGE.

Collection Medium:

Pink top tube 6 mL (K2-EDTA)

Minimum:

5.0 mL Bone Marrow Aspirate in EDTA anti-coagulated pink top tube

Rejection Criteria:

Specimens must be received within 72 hours of collection. Optimal results are achieved if specimen is received within 48 hours of collection.

Delivery Instructions:

Deliver to laboratory immediately after collection.

Turn Around Time:

10–14 days upon receipt in laboratory

Test Limitations:

Samples must be refrigerated within one hour and must be received within 72 hours of collection. Optimal results are achieved if specimen is received within 48 hours of collection.

Methodology:

The NPM1 Mutation Quantitation assay uses multiplex reverse transcription in combination with droplet digital PCR technology. The type A NPM1 duplications occur in most AML cases. The assay can reliably detect 1 tumor cell in a background of 20,000 normal cells. The limit of quantitation is 0.005% NPM1/ABL1 ratio.

CPT Code:

81310