The University of Iowa Carver College of Medicine
Propdium Iodide for Viability
- Propidium Iodide (Invitrogen cat# P1304MP 100mg powder)
- 1X PBS
- 50µg/ml in 1X PBS
Dilute stock 50:1 in cell suspension for final dilution of 1µg/ml (e.g. add 20µl of stock solution to 1ml of cell suspension). Add just before running on flow cytometer.