The University of Iowa Carver College of Medicine

Flow Cytometry Facility

2% Paraformaldehyde Fixative

*Prepare the following protocol in a fume hood.*

To Prepare 1 liter of Fixative

  1. 900ml dd H2O + 500µl 1N NaOH
  2. Heat to 65° - 70°C
  3. While stirring, add 20g paraformaldehyde
  4. Continue heating and stirring until paraformaldehyde is dissolved
  5. Let cool to room temperature
  6. Add 100ml of 10X PBS
  7. pH to 7.3 HCI
  8. Sterile filter
  9. Store at 4°C, protected from light

This reagent is good for at least one month.