Heparin Induced Thrombocytopenia
| Label Mnemonic: | HIT |
| Epic code: | LAB1088 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 86022 |
Specimen(s):
Serum
Collection Medium:
|
| Red top tube 5 mL (Clot Activator) |
Minimum:
1 mL serum
Testing Schedule:
HIT testing is performed daily (holidays and weekends included) at
1300. Sample must be in the lab at that time to be included in the
run for that day.
Reference Range:
Negative
Critical value: Positive
Critical value: Positive
Comments:
For pediatric patients <18 years of age, a Pediatric
Hematology/Oncology Service Consult is required for testing
approval.
It is highly recommended to use the 4T calculator to estimate risk of heparin-induced thrombocytopenia (HIT). Ordering of HIT testing is highly discouraged when the 4T score is 0-3.
Please refer to the Guidelines for Recognition and Management of Heparin Induced Thrombocytopenia which also provides the 4T scoring system.
It is highly recommended to use the 4T calculator to estimate risk of heparin-induced thrombocytopenia (HIT). Ordering of HIT testing is highly discouraged when the 4T score is 0-3.
Please refer to the Guidelines for Recognition and Management of Heparin Induced Thrombocytopenia which also provides the 4T scoring system.
Methodology:
Enzyme Linked Immunosorbent Assay (ELISA) used to detect antibodies
to Platelet Factor 4
Sample Processing:
Centrifuge at a speed and time necessary to get barrier separation
of plasma/serum and cells.
Separate serum/plasma into plastic container and cap (glass is not acceptable).
Label transport tube with two patient identifiers, date and time of collection.
Separate serum/plasma into plastic container and cap (glass is not acceptable).
Label transport tube with two patient identifiers, date and time of collection.
Sample Storage:
Freeze specimen immediately.
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal
bag.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
See also:
Serotonin Release Assay, Unfractionated Heparin, Serum
Serotonin Release Assay, Unfractionated Heparin, Serum